Single-use suspension culture cartridge, 60 mL working volume, pre-sterilized. Continuous perfusion without sparging. For use with the Stämm HTB.
Description
Suspension Cell Cartridge. 60 mL working volume, single-use, pre-sterilized.
Bubble-free suspension culture. Gas exchange by diffusion, not sparging.
Bubble rupture at the gas-liquid interface is the dominant damage mechanism in sparged suspension culture followed by impeller shear. Antifoam and shear protectants (e.g. Pluronic F-68) manage that symptom but with added cost, reduced oxygen transfer, and downstream complications.
The cartridge is built around a gyroid-structured bubble-free bioreactor, its geometry inspired by the efficiency of the human vascular system: a network of microchannels bounded by a gas-permeable, liquid-tight membrane. Gas diffuses through the channel walls. Cells stay in suspension via a controlled rotation cycle. There's no use of impeller or sparger; so no foam is generated.
Continuous perfusion. Fresh medium in, spent medium out, without the metabolite accumulation that defines the back half of a fed-batch run. And no antifoam or shear protectants implies two fewer components in the media formulation and two fewer variables downstream.
The Suspension Cell Cartridge kit includes:
- Bubble-free bioreactor: gyroid microchannel architecture, gas-permeable / liquid-tight
- Formulation consumable: 6 media bags
- Tubing set
- Formulator flask
- 3 inoculation kits
- 3 gas control consumables
- Integrated ports for sampling, inoculation and harvest
- LOT-level traceability
- Single-use, tool-free installation
- 60 mL working volume
- Suitable for suspension lines including CHO and HEK-293
Handling & safety
- Single use only.
- Requires a Stämm HTB Production Unit. One cartridge per run.
- For working volumes and chemical compatibility, see Details.
- Each unit arrives sterile and individually packed.
Details
| Working volume | 60 mL |
| Cell type | Suspension |
| Operating mode | Batch and perfusion |
| Gas exchange | Diffusion (PDMS membrane), no sparging |
| Agitation | None |
| Containment | Self-contained |
| Surface treatment | Custom coating, tailored to the cell line (adherent and suspension supported in the same device) |
| Material | Biocompatible resin, PDMS-coated |
| Animal-origin free | Yes |
| Packaging | Individually packed |
| Traceability | Full traceability by LOT number |
| Shelf life | 1 year |
| Biocompatibility | Passes standard biocompatibility testing |
| Disposal | Autoclave, then discard in a biohazard pouch as pathogenic waste |
| Device requirement | Requires Stämm HTB Production Unit |
| Product code/SKU | BFB-101 |
| Order format | Single-use cartridge, sold in kits of 6 or 12 |
| Country of origin | Argentina |
Every unit includes in-person training on the machine, unrestricted technical maintenance and support, repairs, and any other assistance required to keep the HTB running properly. Support is provided worldwide for the life of the instrument.
Contact support: info@stamm.bio
