The Bioprocessor downsizes a whole biotech facility into an all-in-one, plug & play desktop unit with continuous, unidirectional, and laminar flow. It produces daily harvests and keeps cells at peak efficiency by controlling its microenvironmental parameters, such as culture medium, pH, dissolved oxygen, and cell density, online and inline. It is composed of two main microfluidic devices:
The Cell line on-a-Chip is a replaceable cartridge-type device that provides a constant flow of available cells to continuously inoculate the system.
The Bubble-Free-Bioreactor is an 3D microbioreactor built by additive manufacturing with gas permeable, water tight, and compatible inks. It is composed of hundreds of microchannels that guide cells through the system. These microchannels keep cells in a continuous, unidirectional, laminar flow and allow a perfect mix between cells, media, and gases, keeping them in optimal conditions at all times and enabling them to continually reproduce. The topology of this device responds to cell duplication rate and includes a fresh culture media feeding system that keep cells at the desired concentration.
200-fold decrease in size in comparison to traditional biotech facilities
processing volume decrease between 100x and 400x depending on the cell culture.
the estimated increase in productivity
Biomaterials are one of the key areas for the development of our devices. In the biomaterials team, we are very passionate about discovering new materials for interaction with different cell types. The bio-inks developed by the Biomaterials team do not only have to pursue structural integrity, but they also must be biocompatible to create a suitable environment for living cells. For this purpose, we have a highly qualified group of people experienced in the formulation, analysis, and scaling of biomaterials. One key characteristic of our bio-inks is that they must be photocurable for their use in our 3D printing proprietary technology (Sclereid) to manufacture bioreactors that later are inoculated with cells.
Pulsed Electric Field (PEF) biotechnology applications are used to generate pores in a cell membrane. If the induced pores are small, they can close again (reversible rupture). If the pores are very large, the membrane can no longer repair itself (irreversible rupture) and this results in sterilization. We have investigated and developed a new culture medium sterilization system in continuous mode to feed the Bioprocessor.
Microfluidics is the study and control of small volumes of liquids in microstructures. It allows experimentation with small (micro to nano liters) volumes reducing costs when using expensive reagents. It’s an extremely useful tool in biochemistry and physics. Its implementation requires the design of micro pumps, micro valves, mixers and other micro devices allowing the user to mix, transport, segregate and thermally homogenize fluids. Its advantages are the low reagent consumption, higher fluid control, less waste generation, faster response time and faster, easier and less expensive replication. The complexity requires specialized equipment and a highly skilled workforce.
In laminar flow, the fluid travels smoothly or in regular paths, in contrast to turbulent flow, in which the fluid undergoes irregular fluctuations and mixing.
Laminar flow occurs in cases where the flow channel is relatively small (in the tens of microns), the fluid is moving slowly, and its viscosity is relatively high. The flow of honey through a thin tube or blood flow through a capillary are clear examples of laminar flow.
Crystal Lattices are symmetrical units with three-dimensional patterns which upon being repeated in regular intervals, create Crystallographic structures. These structures are widely studied in diverse areas of science and technology. We are able to print these structures with our innovative 3D printer filling any desired topology, thus creating a network of micro channels that we called Crystallographic Microvasculature.